FIGURE 4.

Effects adding a bc1 complex inhibitor, Antimycin A, on microbial current generation for A. ferrooxidans cultivated in the absence of Fe²⁺ ions at an electrode potential of +0.4 V (vs. SHE). Antimycin A solubilized in methanol (final concentration of Antimycin A is 100 μM) was added into electrochemical reactors at the time points indicated with an arrow (solid line). Methanol lacking Antimycin A was also added into electrochemical reactors as a control experiment (broken line). Initial OD₅₀₀ was 0.02.