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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Jul 31;53(38):10242–10246. doi: 10.1002/anie.201403349

Figure 2.

Figure 2

Cer-TCO localizes and reacts with SiR-Tz to visualize the Golgi in live cells. a) HeLa cells expressing the Golgi reporter protein GalNAcT2-GFP were treated with Cer-TCO (2 μM), subjected to a temperature block to accumulate the ceramide lipid in the Golgi, and labeled with SiR-Tz (2 μM). b–d) Incubation with SiR-Tz alone does not label the cells, whereas treatment with Cer-TCO and Sir-Tz leads to a reaction product (red) that colocalizes with the Golgi marker GalNAcT2-GFP (green); see also Figure S2.