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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Jul 31;53(38):10242–10246. doi: 10.1002/anie.201403349

Figure 3.

Figure 3

a) Endpoint trafficking assay using TfRc-FM4-pH and ‘D/D’ solubilizer to distinguish between cargo that reached the plasma membrane or remained inside the cell. b) HeLa cells expressing TfRc-FM4-pH (green) were treated with or without Cer-TCO and SiR-Tz and fixed 0 or 60 min after the ‘D/D’ solubilizer-promoted release of TfRc-FM4-pH from the ER. Cells were immunostained to visualize the Golgi (magenta) and TfRc-FM4-pH on the cell surface (red). At 0 min, TfRc-FM4-pH (green) is localized to the ER, whereas at 60 min it is localized to the cell surface. Nuclei were stained with Hoechst 33342 (blue). c) Ratio of red/green channels shows the fraction of cargo (TfRc-FM4-pH) that reached the surface.