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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Jul 31;53(38):10242–10246. doi: 10.1002/anie.201403349

Figure 4.

Figure 4

Intra-Golgi trafficking is unaffected by Cer-TCO and SiR-Tz. a) Cells expressing GalNAcT2-GFP (green) are labeled with Cer-TCO and SiR-Tz, photobleached, and the fluorescence recovery after photobleaching monitored vs. time. b) Examples of fluorescence recovery at 0–490 sec after photobleaching cells treated with or without Cer-TCO/SiR-Tz. c) Plots showing that fluorescence recovery occurs at a similar rate and extent irrespective of Cer-TCO/SiR-Tz concentration and with or without temperature block. d) Differential effects of prolonged illumination on GalNacT4-GFP (green) cells labeled with BODIPY-Cer (green) or Cer-TCO and SiR-Tz (red). D = cells dividing; * = dying cell. e) Quantification of live cell data from (d) shows the relative number of healthy cells as a function of time after different conditions.