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. Author manuscript; available in PMC: 2016 Aug 21.
Published in final edited form as: ACS Chem Biol. 2015 Jun 3;10(8):1916–1924. doi: 10.1021/acschembio.5b00367

Figure 3.

Figure 3

Analysis of the ubiquitin proteasome system (UPS). (a) Total ubiquitylation was measured in HEK293 cells with an anti-ubiquitin (Ub) antibody. (b) UbG76VGFP (a Ub green fluorescent protein (GFP) fusion) was stably expressed and measured in HEK293 cells with an anti-GFP antibody. The negative controls used were DMSO and 12. The positive control (+) was generated with MG132 (a proteasome inhibitor).31 All experiments were conducted in triplicate, and exemplary blots are provided. Loading control is given by lamin A.