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Western blot analyses of (a) TCRα–GFP and (b) hemagglutinin (HA)–CD3δ using anti-GFP and anti-HA antibodies, respectively. (c) Analysis of p97 function in HEK293 cells. The unfolded protein response (UPR) was evaluated using an anti-XBP1 antibody to analyze the appearance of spliced XBP1s, lower band. Autophagosome maturation was measured using an anti-LC3 antibody to determine the accumulation of LC3-II, lowest band. Loading controls are given by lamin A or GAPDH.
