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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Xenotransplantation. 2013 Sep 5;20(5):277–291. doi: 10.1111/xen.12047

Figure 1. Workflow for the discovery of human and porcine glycans.

Figure 1

A. This study compares the N-linked glycomes of humans, domestic pigs, GGTA1 knockout pigs and GGTA1/CMAH knockout pigs. B. Serum samples were treated with the deglycosylating enzyme, PNGaseF, to remove N-linked glycans from serum proteins. C. Native glycans were stabilized by reduction and permethylation on solid-phase sodium hydroxide spin columns. D. Collision induced dissociation of permethylated glycans resulted in predictable fragmentation that was analyzed using the nomenclature of Domon and Costello (19).