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MALDI-TOF MS profile of ion 1595.8, Man5. Incomplete permethylation results in predictable ions 14 Da downstream of a fully permethylated ion. The black arrow points to the absence of ion 1581.8 m/z, −14 Da from ion 1595.8 m/z. The grey arrow indicates the absence of ion 1611.8 m/z, a +16 Da ion that could originate from the formation of a potassium adduct instead of the more common sodium adduct.
