Figure 5.
MALDI-TOF/TOF MS fragmentation profiles for the reduced and permethylated N-linked glycans of the GGTA1/CMAH knockout pig. The ions 1595.7 (A), 1851.8 (B), 2245.8 (C) and 2982.2 (D) were more abundant in the GGTA1/CMAH knockout pigs samples. Major peaks were annotated with their proposed structures based on the proposed carbohydrate structures using the nomenclature of the Consortium for Functional Glycomics (18). The complete N-linked glycan structure is shown to the left of the MS spectra.