Fig 1. Schematic representation of recombinant lentiviral TRIP/JEV vectors.
The organization of JEV genomic RNA is shown in top. The codon-optimized sequence encoding prM and E from JEV G3 strain RP-9 was cloned into a TRIP lentiviral vector under the control of human cytomegalovirus immediate early promoter (PCMVie). The TRIP/JEV.prME vector includes the signal peptide sequence of prM (SP) followed by the entire prM and E gene regions of JEV strain RP-9 of G3. The TRIP/prMEΔTM vector includes the same JEV sequence except that E was deleted from its two transmembrane domains TMD1 and TMD2.