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. 2015 Oct 5;9(10):e0004081. doi: 10.1371/journal.pntd.0004081

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© 2015 de Wispelaere et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — In (A), immunofluorescence analysis of transduced cells using the anti-E mAb 4G2 as primary antibody. In (B, C), immunoblot analysis of prM and E accumulation in lysates (B) or supernatants (C) of cells transduced with TRIP/JEV vectors. TRIP/GFP vector served as a control. The intracellular prM and E were detected with a mouse polyclonal serum directed against JEV strain RP-9 (JEV antiserum). Detection of calnexin (CNX) served as a loading control. JEV E and prM in supernatants of transduced cells were detected with mAb 4G2 or with the JEV antiserum, respectively. JEV VLPs were purified from supernatants of TRIP/JEV vector-transduced cells and analyzed by immunoblotting using anti-E mAb 4G2 and JEV antiserum. TRIP/GFP served as a negative control. The bands corresponding to prM, E or E^ΔTM and CNX are indicated with arrows to the right of the blots.