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. 2015 Oct 5;9(10):e0004081. doi: 10.1371/journal.pntd.0004081

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© 2015 de Wispelaere et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Lysates obtained from SK-N-SH cells infected with JEV strain RP-9 (JEV) or mock-infected (m.i.) were tested with pooled immune sera (antisera) from BALB/c mice twice inoculated with 5 log₁₀ TU of TRIP/JEV.prME or TRIP/JEV.prME vector by immunoblot assay. TRIP/JEV antisera were collected 3 weeks after the boosting inoculation. Mouse polyclonal serum directed against JEV strain RP-9 of G3 (JEV antiserum) served as a positive control. The bands corresponding to JEV E, NS1/NS1’ and prM proteins are indicated with arrows to the left of the blot.