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. 2015 Oct 5;10(10):e0139456. doi: 10.1371/journal.pone.0139456

Fig 6. Analysis of the role of miR–25 in the regulation of SOCS4 expression and IL-23-mediated thyroid cancer cell migration and invasion.

Fig 6

(A) K1 cells were transfected with the indicated plasmid, then treated with rhIL–23 (50 ng/ml) for 48 h. SOCS4 mRNA (left panel) and protein (right panel) were detected by qRT-PCR and western blot, respectively. (B) Cells were transfected with miR–25 inhibitor or control, and the experiments were performed as in A. (C, D) K1 cells were transfected with miR–25 mimic (C) or inhibitor (D) and controls, then treated with rhIL–23 (50 ng/ml) for 48 h and allowed to migrate towards serum for 24 h. (E, F) Cell invasion assay experiments were performed with the same conditions as in C and D. (G, H) Wound healing assay experiments were performed with the same conditions as in C and D. All the experiments were repeated at least 3 times with similar results. Bar graphs represent mean ± SD, n = 3 (**P < 0.01; *P < 0.05).