Table 1. Overview of ASO experiments.
| Gene name (Mutant name) | Expression pattern | No. of effective ASOs/designed | Assay of ASO knockdown efficiency | Reference for published morphology |
|---|---|---|---|---|
| PROTEIN-CODING | ||||
| alk8 (lost-a-fin) | maternal + zygotic | 2/2 | morphology; RT-PCR | [38,39] |
| bmp2b (swirl) | zygotic | 0/2 | morphology; RT-PCR | [50–53] |
| bmp7a (snailhouse) | zygotic | 2/2 | morphology; RT-PCR | [34,35,53] |
| chordin (dino) | zygotic | 2/2 | morphology; RT-PCR | [6,36,54] |
| ctnnb2 (ichabod) | maternal | 1/2 | morphology (ventralization) | [55] |
| cx41.8 (leopard) | expressed in late larvae + adults | 0/3 | morphology (spotted pigment pattern) | [56] |
| dnd/dead-end | germ-cell specific | 1/4; 1 * /4 | morphology (ablation of germ cells at 24 hpf) | [42,43] |
| hcrt/hypocretin | expressed from 1dpf | 0/3 | in situ (hcrt expressing cells) | [57] |
| mitfa (nacre) | expressed from 1dpf | 0/2 | morphology; RT-PCR | [6,58] |
| oep/one-eyed pinhead | maternal + zygotic | 2/5 | morphology; RT-PCR | [28,59,60] |
| slc24a5 (golden) | expressed from 1dpf | 0/3 | morphology (loss of pigment) | [61] |
| smad2 | maternal + zygotic | 2/3 | morphology; RT-PCR | [62] |
| smad5 (somitabun) | maternal + zygotic | 2/2 | morphology; RT-PCR | [40,53] |
| ta-T/ntla/no-tail a | zygotic | 2 * /2 | morphology; RT-PCR | [6,41] |
| toddler/apela/ELABELA | zygotic | 0/2 | morphology (gastrulation + heart defect) | [63,64] |
| tll1/tolloid/mini fin | zygotic | 0/2 | morphology; RT-PCR | [53,65] |
| wnt11 (silberblick) | zygotic | 2 * /2 | morphology; RT-PCR | [53,66] |
| wnt5b (pipetail) | zygotic | 0/2 | morphology; RT-PCR | [53,67] |
| NON-CODING | ||||
| MALAT1 | highly expressed from 1 dpf | 2/3 | RT-PCR | |
| miR-126 | expressed from 1 dpf; endothelia | 0/2 | morphology (blood formation) | [68,69] |
*partial knockdown effect
For list of all ASO sequences, including information regarding effectiveness, see S1 Text.