Table 2. Primers used for pyrosequencing.
Primer | Sequence (5’-3’) | Genomic co-ordinates (GRCh38/hg38) |
---|---|---|
Forward | GGGGGTTGGGGGATATAAA | chr13:91348926–91348944 |
Reverse (biotinylated) | CCTTTTTCAATTCCTTTTCCCTTTAC | chr13:91349234–91349209 |
Sequencing | GGGGGATATAAAGGAG | chr13:91348933–91348948 |