Fig 3. The Tie1 T794 mutant disrupts endothelial tube formation.
a Representative images of HUVECs left uninfected or infected with adenoviruses encoding GFP, Tie1-WT, or Tie1-T794A, plated on Matrigel and allowed to form tubes for 6 hours. b, c Quantification of endothelial tube networks in vitro. Cells expressing the T794A mutant formed significantly shorter tubes (b; *, multiple comparisons P≤ 0.02) with fewer nodes and branches (c; *, multiple comparisons P< 0.01) (n = 11 independent experiments with 4 or more images per experiment).