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. 2015 Oct 5;10(10):e0138833. doi: 10.1371/journal.pone.0138833

Fig 5. Effect of R568 and CaSR agonists on calcium deposition and osteogenic markers in arterial explants.

Fig 5

Medium sized arterial explants from healthy individuals donating a kidney (control, n = 9) and patients with CKD (n = 11) undergoing a kidney transplant were cultured for 7 days. Shaded boxes represent exposure to R568 (100nmol/L). A) CaSR and B) Runx2 expression in Control and CKD artery (n = 4 per sample). CaSR expression was significantly lower in CKD patients (p = 0.0003), whereas Runx2 was significantly higher (p = 0.008). C) Total artery calcium content was not significantly different between control and CKD patients, and not significantly altered by R568. Expression of D) Runx2 (p = 0.0003) and E) DMP–1 (p = 0.0003) was significantly higher in CKD arteries compared to controls. R568 exposure did not significantly alter expression of these proteins. DMP–1 –dental matrix protein–1; CaSR–calcium-sensing receptor.