Table 2. The Hotelling’s T-squared test of VMR of the same strain at 6 dpf.

A. The Hotelling’s T-squared test of VMR of the same strain at 6 dpf before and after light change
Comparison	Test statistic (p-value)
	Strain: AB	Strain: TL	Strain: TLAB
Light-On stimulus (Before light change VS. After light change)	15.845 (< 0.0001)	8.3863 (< 0.0001)	14.595 (< 0.0001)
Light-Off stimulus (Before light change VS. After light change)	26.881 (< 0.0001)	11.784 (< 0.0001)	14.984 (< 0.0001)
B. The Hotelling’s T-squared test of VMR of the same strain at 6 dpf before and after light change using data only from the first technical repeat
	Strain: AB	Strain: TL	Strain: TLAB
Light-On stimulus (Before light change VS. After light change)	2.8817 (< 0.0001)	3.3889 (< 0.0001)	5.6767 (< 0.0001)
C. The Hotelling’s T-squared test of VMR of the same strain at 6 dpf before and after light change using data from the second and third technical repeats
	Strain: AB	Strain: TL	Strain: TLAB
Light-On stimulus (Before light change VS. After light change)	16.534 (< 0.0001)	6.8506 (< 0.0001)	11.255 (< 0.0001)