Table 5. The Hotelling’s T-squared test of Light-On VMR between different stages of the same strain.

A. The Hotelling’s T-squared test of Light-On VMR between different stages of the same strain
Comparison	Test statistic (p-value)
	AB (-29–0 s)	AB (1–30 s)	TLAB (-29–0 s)	TLAB (1–30 s)
3 dpf VS. 6 dpf	17.851 (< 0.0001)	13.494 (< 0.0001)	13.617 (< 0.0001)	12.944 (< 0.0001)
6 dpf VS. 9 dpf	1.6319 (0.0124)	12.293 (< 0.0001)	4.1654 (< 0.0001)	16.358 (< 0.0001)
3 dpf VS. 9 dpf	13.147 (< 0.0001)	17.398 (< 0.0001)	11.594 (< 0.0001)	35.358 (< 0.0001)
B. The Hotelling’s T-squared test of Light-On VMR between different stages of the same strain using data only from the first technical repeat
	AB (-29–0 s)	AB (1–30 s)	TLAB (-29–0 s)	TLAB (1–30 s)
3 dpf VS. 6 dpf	5.9776 (< 0.0001)	4.1605 (< 0.0001)	5.0116 (< 0.0001)	7.4399 (< 0.0001)
6 dpf VS. 9 dpf	1.406 (0.0396)	6.1705 (< 0.0001)	1.7169 (0.0046)	7.2368 (< 0.0001)
3 dpf VS. 9 dpf	6.7456 (< 0.0001)	5.9348 (< 0.0001)	4.4079 (< 0.0001)	16.002 (< 0.0001)
C. The Hotelling’s T-squared test of Light-On VMR between different stages of the same strain using data from the second and third technical repeats
	AB (-29–0 s)	AB (1–30 s)	TLAB (-29–0 s)	TLAB (1–30 s)
3 dpf VS. 6 dpf	15.208 (< 0.0001)	11.384 (< 0.0001)	10.943 (< 0.0001)	9.1793 (< 0.0001)
6 dpf VS. 9 dpf	1.3349 (0.073)	8.1946 (< 0.0001)	3.5779 (< 0.0001)	13.047 (< 0.0001)
3 dpf VS. 9 dpf	9.7053 (< 0.0001)	15.538 (< 0.0001)	10.421 (< 0.0001)	25.398 (< 0.0001)