Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 5;10(10):e0139454. doi: 10.1371/journal.pone.0139454

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ikai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 1 — (A) Schematic diagram of the mfa gene cluster in P. gingivalis ATCC 33277, based on sequence NC_010729 deposited in NCBI. The cluster contains five genes, mfa1-5, in the same transcriptional orientation. (B) Mfa4 expression. Whole-cell lysates from P. gingivalis JI-1 (lane 1), FMFA4 (lane 2), and FMFA4C (lane 3) were separated by SDS-PAGE, immunoblotted, and probed with Mfa4 antiserum. (C) Subcellular localization. A culture of P. gingivalis JI-1 was harvested by centrifugation. The culture supernatant (CS) was collected and proteins were concentrated by ammonium sulfate precipitation. Whole-cell lysates (W), obtained by lysing cells through French press, were fractionated into soluble (Sol), envelope (Env), inner membrane (IM), and outer membrane (OM) fractions. Mfa1 fimbriae (F) were purified from the soluble fraction and used as positive control. Samples were separated by SDS-PAGE and subjected to immunoblotting using anti-Mfa4 as probe.