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. 2015 Oct 5;10(10):e0139454. doi: 10.1371/journal.pone.0139454

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© 2015 Ikai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Culture supernatants (CS) and whole-cell lysates (W) from JI-1, FMFA4, and FMFA4C were separated by SDS-PAGE and probed with Mfa5 antibodies by immunoblotting. Structurally intact Mfa1 fimbriae purified from JI-1 (F), which contains Mfa5, were used as positive control. Possible Mfa5 degradation products with molecular weight 100 and 78 kDa are marked with *.