. 2015 Oct 5;10(10):e0139454. doi: 10.1371/journal.pone.0139454

Table 1. Bacterial strains and plasmids.

Strain or plasmid	Genotype and relevant description ¹	Source
*Porphyromonas gingivalis*
ATCC 33277	Type strain, Gm^r	ATCC ²
JI-1	fimA deletion mutant from ATCC 33277, Cm^r	[12]
JI-12	mfa2-deficient mutant from JI-1, Cm^r Em^r	[12]
FMFA4	mfa4-deficient mutant from JI-1, Cm^r Em^r	This study
FMFA4C	FMFA4 complemented with mfa4 through pTCOW::ragAP::mfa4, Cm^r Em^r Tc^r	This study
KDP98	fimA-deficient mutant from ATCC 33277, Em^r	[20]
KDP112	rgpA/B-deficient mutant from ATCC 33277, Tc^r Em^r	[21]
SMF-fimA	fimA and mfa1 double mutant from ATCC 33277, Cm^r Em^r	[13]
FMFA3	mfa3 deletion mutant from KDP98, Cm^r Em^r	[13]
FMFA5	mfa5-deficient mutant from JI-1, Cm^r Em^r	This study
*Escherichia coli*
TOP10	F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Str^R) endA1 nupG λ-	Thermo Fisher Scientific Inc.
S17-1	Used to deliver pT-COW to Bacteroides and P. gingivalis via conjugation	[22]
Plasmids
pVA2198	Source of the drug cassette ermF-ermAM, Em^r	[23]
pCR-Blunt II-TOPO	Cloning vector, Km^r	Invitrogen
pTCOW::ragAP	pTCOW derivative containing a ragA promoter; Ap^r in E. coli, Tc^r in P. gingivalis	[24]
pTCOW::ragAP::mfa4	pTCOW::ragAP derivative containing mfa4 ORF; Ap^r in E. coli, Tc^r in P. gingivalis	This study

¹ Ap^r, ampicillin resistance; Cm^r, chloramphenicol resistance; Em^r, erythromycin resistance; Gm^r, gentamicin resistance; Km^r, kanamycin resistance; Tc^r, tetracycline resistance; Str^r, streptomycin resistance.

² ATCC, American Type Culture Collection.