Figure 5.

Leishmania amazonensis reduces the phosphorylation levels of p105 through inhibition of GSK3β and inactive PTEN in infected macrophages. THP-1 cells were differentiated into macrophages and infected with promastigotes of L. amazonensis, as indicated. The protein extracts were obtained and subjected to Western blot using (a) the anti-p105 antibodies phosphorylated at serine 907 residue and anti-p105, (b) anti-GSK3β phosphorylated at serine 9 residue and (c) anti-PTEN phosphorylated at serine 370 residue. Anti-β-actin and anti-lamin A/C were used as loading control.