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. 2015 Sep 23;5(9):150118. doi: 10.1098/rsob.150118

Figure 6.

Figure 6.

Akt 1 knocked-down expression impairs NF-κB-p50 activation in infected macrophages. (a) Western blot of total protein extract obtained from differentiated THP-1 stably knocked-down for Akt 1 expression. Monocytic THP-1 were not transduced or transduced with lentivirus carrying the construction: shpLVTHM, shScr (Scrambled), shAkt 1 (1) and shAkt 1 (2). (b) Western blot of nuclear protein extract from differentiated THP-1 knocked-down for Akt 1 (shAkt 1 (1)) and infected with L. amazonensis for 5 h. Relative levels of nuclear p50, calculated by densitometry and normalized with the endogenous control lamin A/C, are indicated at bottom. (c) Human primary macrophages and differentiated THP-1 were infected with metacyclic promastigote of L. amazonensis and treated with Akt 1/2 inhibitor. Differentiated THP-1 knocked-down for Akt 1 was infected with L. amazonensis metacyclic promastigote. After 72 h, the infection index was evaluated as described in Material and methods. *p < 0.05.