Table 2.
Mitochondrial primers [40] and microsatellite primers characterized by Fitzsimmons et al. [14] tested for use in identifying hybrids between C. acutus and C. moreletii. (Fixed marker indicates alleles unique to parental species. Variable marker indicates frequency variation in alleles between parental species.)
| primer name | primer sequence (5′–3′) | species | repeat motif | amplification | fixed | variable | |
|---|---|---|---|---|---|---|---|
| mtDNA | 12s | F: CCGTCTTTGACAGTC | |||||
| R: ATGTTCCAAGCACACCTTCC | |||||||
| 16s | F: AAGGTAGCGTAATCATTTG | ||||||
| R: GGGGATTGCGCTGTTATCCCTG | |||||||
| Dloop | F: GCCGACATTCTTATTAAACTAC | ||||||
| R: GCAGATAAATGAATGCCTTAT | |||||||
| microsatellite | Cj119 | F: GTTTGCTGTGGAATGTTTCTAC | C. johnsoni | (CA)14 | yes | yes | yes |
| R: CGCTATATGAAACGGTGGCTG | |||||||
| C391 | F: ATGAGTCAGGTGGCAGGTTC | C. acutus | (CA)22 | yes | no | yes | |
| R: CATAAATACACTTTTGAGCAGCAG | |||||||
| Cj104 | F: TCCTTCCATGCATGCACGTGTG | C. johnsoni | (CA)12 | yes | yes | yes | |
| R: GTTTCAGTGTCTGGTATTGGAGAAGG | |||||||
| Cj105 | F: CAACAGAAAGTGCCACCTCAAG | C. johnsoni | (CA)14 | multiple bands | no | no | |
| R: GTTTGATTATGAGACACCGCCACC | |||||||
| Cj107 | F: ACCCCGCATTCTGCCAAGGTG | C. johnsoni | (CA)16 | multiple bands | no | no | |
| R: GTTTATTGCCATCCCCACTGTGTC | |||||||
| Cj122 | F: GTTTCATGCTGACTGTTTCTAATCACC | C. johnsoni | (CA)ls | yes | mono | mono | |
| R: GGAACTACAATTGGTCAACCTCAC | |||||||
| Cj127 | F: CCCATAGTTTCCTGTTACCTG | C. johnsoni | (CT)7TT(CT)12 (CA)16 | yes | no | yes | |
| R: GTTTCCCTCTCTGACTTCAGTGTTG | |||||||
| Cj128 | F: ATTGGGGCAGATAAGTGGACTC | C. johnsoni | (CA)22 | no | no | no | |
| R: GTTTCTGCTTCTCTTCCCTACCTGG | |||||||
| Cj35 | F: GTTTAGAAGTCTCCAAGCCTCTCAG | C. johnsoni | (CT)7TA(CA)17(CT)12 | yes | yes | yes | |
| R: CTGGGGCAAGGATTTAACTCTC | |||||||
| Cj101 | F: ACAGGAGGAATGTCGCATAATTG | C. johnsoni | (CA)12 | yes | no | yes | |
| R: GTTTATACCGTGCCATCCAAGTTAG | |||||||
| Cj131 | F: GTTTGTCTTCTTCCTCCTGTCCCTC | C. johnsoni | (CA)14 | yes | yes | yes | |
| R: AAATGCTGACTCCTACGGATGG | |||||||
| Cjl6 | F: CATGCAGATTGTTATTCCTGATG | C. johnsoni | (CA)20 | yes | unknown | unknown | |
| R: TGTCATGGTGTCAATTAAACTC | |||||||
| Cjl8 | F: ATCCAAATCCCATGAACCTGAGAG | C. johnsoni | unpublished | yes | yes | yes | |
| R: CCGAGTGCTTACAAGAGGCTGG | |||||||
| Cp10 | F: GATTAGTTTTACGTGACATGCA | C. porosus | (CA)ls | yes | mono | mono | |
| R: ACATCAAGTCATGGCAGGTGAG | |||||||
| CUD68 | F: GCTTCAGCAGGGGCTACC | C. acutus | (CA)13 | only C. acutus | plus/minus | no | |
| R: TGGGGAAACTGCACTTTAGG | |||||||
| CUC20 | F: GATCTGCAGTGCAAGAAAG | C. acutus | unpublished | yes | yes | yes | |
| R: GGTTTAGCGGTCACAGTAAC | |||||||
| CUD78 | F GAAGTGAATGCCATCTATCA | C. acutus | (CA)15 | yes | mono | mono | |
| R AATTGCATCCCCTTTTG | |||||||
| CUI 108 | F: ACTGGCCACAGCTGGGGTA | C. acutus | (CA)20 | multiple bands | no | no | |
| R: CCAGCAGCGTGGAGAGCTG |