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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2015 Apr 30;68:120–130. doi: 10.1016/j.mcn.2015.04.005

Figure 1. Membrane projection containing DAT are filopodia.

Figure 1

(A) PAE/RFP-HA-DAT cells were fixed, stained with phalloidin-Alexa680, permeabilized and immunostained with rabbit polyclonal MyoX antibodies followed by secondary antibody conjugated to Alexa488. 3D images were acquired through 488 (green, MyoX), 561 (red, DAT-RFP) and 640 (cyan, actin) channels. Maximal projection of the z-stack is presented. Insets show high magnification of the region marked by white rectangles.

(B) GFP-MyoX was transiently expressed in PAE/RFP-HA-DAT cells, and 3D live-cell imaging was performed through 488 (green, GFP) and 561 (red, RFP) channels as described in “Methods”. A single x–y confocal image of merged GFP and RFP fluorescence is presented.

Scale bars, 10 μm.