. Author manuscript; available in PMC: 2016 Sep 1.

Published in final edited form as: Mol Cell Neurosci. 2015 Jun 16;68:177–185. doi: 10.1016/j.mcn.2015.06.008

Table 1.

Molar Ratios of Neuropeptides Released by Secretagogue Stimulation

Secretagogue	(Met)enkephalin	Galanin	NPY	VIP	Molar Ratio.

	fmol/well				VIP/NPY/Gal/Enk

KCl	2064.9 ± 577.9	45.2 ± 9.9	13.4 ± 1.3	10.0 ± 0.7	1.0/1.3/5.0/207
Nicotine	3899.8 ± 451.6	49.1 ± 5.8	11.2 ± 0.8	8.3 ± 2.3	1.0/1.3/6.0/471
Carbachol	4655.8 ± 780.7	68.8 ± 6.8	30.7 ± 2.0	13.3 ± 2.1	1.0/2.0/5.0/350
PACAP	1055.1 ± 447.0	36.9 ± 12.9	3.3 ± 0.6	5.4 ± 1.1	1.0/0.6/7.0/196
Bradykinin	538.3 ± 450.7	33.3 ± 13.0	3.9 ± 1.4	7.3 ± 1.1	1.0/0.5/5.0/74
Histamine	1196.8 ± 664.4	34.6 ± 15.7	2.0 ± 1.6	5.9 ± 0.9	1.0/0.4/6.0/204

Secretagogue stimulation of neuropeptide secretion from chromaffin cells was measured for (Met)enkephalin, galanin, NPY and VIP (stimulated amounts of neuropeptides released into the culture media minus basal secretion of each of these neuropeptides). Results are shown as mean ± s.e.m. (n = 3). Molar ratios of secreted neuropeptides are illustrated for VIP/NPY/Gal/Enk (Gal, galanin; Enk, (Met)enkephalin). The average molar ratio for all secretagogues was 1.0/1.02/5.7/250.