Table 2.
Secretagogue | Dopamine | Norepinephrine | Epinephrine | Molar Ratio |
---|---|---|---|---|
. | pmol/well | DA/NE/Epi | ||
KCl | 216.8 ± 25.5 | 4736.0 ± 424.7 | 8 753.5 ± 846.4 | 1/22/40 |
Nicotine | 314.2 ± 21.1 | 6026.6 ± 338.4 | 12373.5 ± 666.5 | 1/19/40 |
Carbachol | 404.5 ± 13.6 | 7092.2 ± 141.4 | 15294.6 ± 696.5 | 1/17/38 |
PACAP | 113.5 ± 11.9 | 2373.9 ± 207.7 | 5324.6 ± 311.1 | 1/20/50 |
Bradykinin | 48.1 ± 10.8 | 1497.0 ± 185.9 | 4074.3 ± 295.1 | 1/31/84 |
Histamine | 117.4 ± 11.9 | 1592.5 ± 159.3 | 6039.9 ± 252.3 | 1/14/51 |
Secretagogue stimulation of catecholamine secretion from chromaffin cells was measured for dopamine (DA), norepinephrine (NE), and epinephrine (Epi) (stimulated amounts of neuropeptides released into the culture media minus basal secretion of each of these catecholamines). Results are shown as mean ± s.e.m. (n = 3). Molar ratios of secreted catecholamines are illustrated for DA/NE/Epi. The average molar ratio for all secretagogues tested was 1.0/20.5/50.5.