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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2015 Jun 16;68:177–185. doi: 10.1016/j.mcn.2015.06.008

Table 3.

Content of neuropeptide and catecholamine neurotransmitters in large dense core secretory vesicles (LDCSV) isolated from bovine adrenal medulla.

A. Neuropeptides
Neuropeptide Concentration (fmol/μg) Molar Ratio, Normalized
Met-enkephalin 375 ± 30.7 123,333
Galanin 0.13 ± 0.006 43
NPY 63 ± 9.3 27,000
VIP 0.004 ± 0.0009 1
B. Catecholamines
Catecholamine Concentration (fmol/μg) Molar Ratio, Normalized
Dopamine 7.5 ± 8.9 1.0
Norepinephrine 27.0 ± 2.0 3.6
Epinephrine 35.5 ± 5.6 4.7

LDCSV were isolated from chromaffin cells of bovine adrenal medulla and neurotransmitters were assayed as described in the methods, for neuropeptides (panel A) and catecholmines (panel B). Neurotransmitter concentrations were determined in three preparations of isolated LDCSV, and averages ± s.e.m. are expressed as fmol neurotransmitter per μg protein. Molar ratios of neuropeptides were calculated by normalization to VIP as ‘1’. Molar ratios of catecholamines were calculated by normalization to dopamine as ‘1.0.’