Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2015 Aug 5;68:234–243. doi: 10.1016/j.mcn.2015.08.003

Figure 1.

Figure 1

A) Comparison of Thr286 phosphorylation in purified CaMKIIα autophosphorylated in vitro and CaMKIIα in whole lysates of dorsal striatum collected after freezing slices. Numbers below each lane of the blot indicate the P-T286/total signal ratio. Thr286 autophosphorylation of purified CaMKIIα (1 μM) saturates with ATP concentration higher than 2 μM. Notably, CaMKIIα in these striatal samples appears to be Thr286 phosphorylated to similar saturating levels based on the P-T286/total ratio. B) Representative immunoblots of whole lysates of dorsal striatal punches collected either from flash frozen slices on dry ice (frozen) or from slices chilled on regular ice (cold). Quantitation of P-T286 and total CaMKIIα signals from 6 and 5 slices, respectively, revealed that Thr286 phosphorylation in slices dissected on ice is approximately 15% of the levels detected from frozen slices (below). Significance determined by unpaired Student's ttest (***p<0.001).