Figure 2.
Striatal CaMKII is regulated by membrane depolarization or by selective activation of LTCCs. Brain slices were incubated with (light/dark gray bars) or without (white bars): A) 40 mM KCl for 1 min (light gray) or nimodipine (10 μM, >25 min) followed by 40 mM KCl for 1 min (dark gray). B) 500 nM FPL64176 for 2 min. C) 5 μM BayK8644 for 2 min. Whole lysates of dorsal striatum were immunoblotted for P-Thr286 and total CaMKIIα. Representative blots are shown above bar graphs reporting P-T286/total CaMKIIα ratios as the mean ± SEM for the indicated number of replicates. Significance determined by ANOVA followed by Tukey post-test (A) or by unpaired Student's -test (B,C) (*p<0.05).