Table 1.
Real-time RT-PCR primers used in study and the amplified product length
| Gene | Accession number | Primer sequences (5′–3′) | Product length |
|---|---|---|---|
| TNF-α | NM_012675 | Sense: AGA ACA GCA ACT CCA GAA CAC CCT | 148 |
| Antisense: ATC TCG GAT CAT GCT TTC CGT GCT | |||
| IL-6 | NM_012589 | Sense: ATA TAC CAC TTC ACA AGT CGG | 151 |
| Anti-sense: GGC AAA TTT CCT GGT TAT ATC C | |||
| IL-10 | NM_012854 | Sense: GAC CAG CTG GAC AAC ATA CT | 143 |
| Antisense: GAG GGT CTT CAG CTT CTC TC | |||
| COX-2 | NM_017232 | Sense: TCC AGT ATC AGA ACC GCA TTG CCT | 149 |
| Antisense: AGC AAG TCC GTG TTC AAG GAG GAT | |||
| HO-1 | NM_012580 | Sense: AAC AAG CAG AAC CCA GTC TAT GC | 212 |
| Antisense: GGC CCA CGC ATA TAC CCG CTA CCT | |||
| β-Actin | NM_031144 | Sense: CTA CAA TGA GCT GCG TGT GGC | 229 |
| Antisense: CAG GTC CAG ACG CAG GAT GGC |
COX, cyclooxygenase; HO, heme oxygenase; IL, interleukin; RT-PCR, reverse transcription-polymerase chain reaction; TNF-α, tumor necrosis factor alpha