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. 2015 Mar 28;39(4):354–364. doi: 10.1016/j.jgr.2015.03.006

Fig. 5.

Fig. 5

Effect of total saponin from Korean Red Ginseng (KRG-TS) on inositol 1,4,5-trisphosphate receptor type I (IP3RI) (Ser1756) phosphorylation. Lane 1, intact platelets (base); Lane 2, thrombin (0.05 U/mL); Lane 3, thrombin (0.05 U/mL) + KRG-TS (100 μg/mL); Lane 4, thrombin (0.05 U/mL) + KRG-TS (150 μg/mL); Lane 5, thrombin (0.05 U/mL) + KRG-TS (150 μg/mL) + Rp-8-Br-cAMPS (250μM); Lane 6, thrombin (0.05 U/mL) + KRG-TS (150 μg/mL) + Rp-8-Br-cGMPS (250μM); Lane 7, thrombin (0.05 U/mL) + 8-(4-chlorophenylthio)-cyclic adenosine monophosphate (pCPT-cAMP; 1mM); and Lane 8, thrombin (0.05 U/mL) +8-Br-cGMP (1mM). Western blotting was performed as described in the “Materials and methods” section. Data are expressed as the mean ± standard deviation (n = 4). *p < 0.05 versus the thrombin-stimulated platelets, **p < 0.05 versus the thrombin-stimulated human platelets in the presence of KRG-TS (150 μg/mL).