Effect of NBCe1 deletion on urinary parameters. Top: effects on urine ammonia. Despite the associated metabolic acidosis with NBCe1 gene deletion, ammonia excretion decreased with heterozygous gene deletion and was suppressed significantly further with homozygous deletion. n = 26, 44, and 16 for wild-type, heterozygous, and homozygous NBCe1 deletion, respectively. Bottom: effects on urine pH. Urine pH was significantly more acidic (lower) in mice with heterozygous NBCe1 deletion than in wild-type mice and was decreased significantly further by homozygous NBCe1 deletion. n = 18, 29, and 11 for wild-type, heterozygous, and homozygous NBCe1 deletion, respectively.