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Effect of NBCe1 deletion on phosphate-dependent glutaminase (PDG) expression. Top: effects of NBCe1 deletion on PDG expression. Middle: expression of the “housekeeping” protein β-actin in the same blot showing that changes in PDG expression were not due to changes in protein loading or transfer. Bottom: quantitative analysis of PDG expression relative to β-actin expression. NBCe1 deletion resulted in significant inhibition of PDG expression. n = 7 for each genotype. *P < 0.05.
