Fig. 5.

Effect of NBCe1 deletion on phosphoenolpyruvate carboxykinase (PEPCK) expression. Top: effects of NBCe1 deletion on PEPCK expression by immunoblot analysis. Heterozygous NBCe1 deletion significantly decreased PEPCK expression despite the associated metabolic acidosis. Homozygous NBCe1 deletion resulted in more marked loss of PEPCK expression. n = 7 for each genotype. Bottom: effects of NBCe1 deletion on PEPCK expression by immunohistochemistry. Compared with PEPCK immunolabel in wild-type mice, there was very weak PEPCK immunolabel in the proximal tubule of homozygous NBCe1 deletion (KO) mice.