Fig. 6.

Effect of NBCe1 deletion of glutamine synthetase expression. Top: effects of NBCe1 on glutamine synthetase expression by immunoblot analysis. Heterozygous NBCe1 deletion significantly increased glutamine synthetase expression, and homozygous deletion (KO) increased expression significantly further. This was in contrast to the expected inhibition of glutamine synthetase expression in response to metabolic acidosis. n = 7 for each genotype. Bottom: representative immunohistochemistry of glutamine synthetase expression in the cortex. Homozygous deletion (KO) increased glutamine synthetase immunolabel in proximal tubule segments.