Fig. 7.

Na⁺/H⁺ exchanger 3 (NHE3) expression and the effect of NBCe1 deletion. Top: immunoblot analysis of NHE3 expression in kidneys from mice with intact (Wt), heterozygous NBCe1 deletion (Het), and homozygous NBCe1 deletion (KO). The apparent molecular mass for mouse NHE3 of ∼75 kDa was similar to that which we and others have previously reported (33, 36, 40). Middle: expression of the housekeeping protein β-actin in the same blot showing that differences in NHE3 expression were not due to differences in protein loading or transfer. Bottom: quantitative analysis. NBCe1 deletion caused a trend for a gene dose-related decrease of NHE3 expression, but the differences were not statistically significant (NS).