IGF1R loss does not alter measures of colon growth in lean or obese mice, and neither obesity nor IGF1R deletion impacts IR protein levels in CECs. A–C: colon weight, length, and crypt depth. Values are means ± SE (n = 6 WT-Lean, 4 WT-Obese, 5 VC-Lean, and 4 VC-Obese for colon weight and length; n = 7 WT-Lean, 6 WT-Obese, 6 VC-Lean, and 5 VC-Obese for crypt depth). D: representative Western blot showing IR protein in isolated CECs of lean and obese WT-IGF1Rfl/fl and VC-IGF1RΔ/Δ animals and quantification of IR protein levels relative to β-actin. Values are means ± SE (n = 3 WT-Lean, 4 WT-Obese, 3 VC-Lean, and 3 VC-Obese). aP < 0.05 vs. lean of the same genotype (by 2-way ANOVA with Tukey's multiple-comparisons test); ns, no significance.