Fig. 3.

LA-CS increases apical surface MCT1 level via decreasing endocytic internalization. A: Caco-2 monolayers (14-day postplating) were treated with LA-CS for 3 h, and apical membrane MCT1 levels were measured by cell surface biotinylation. B: following surface biotinylation, Caco-2 monolayers were incubated with LA-CS at 37°C for 3 h, and endocytosed MCT1 fractions were measured by reversible biotinylation, as described in materials and methods. Representative blots of 3 independent experiments are shown in A and B.