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Spleen cells were stimulated with PMA and ionomycin and were collected from both WT and PKU mice fed either the casein or GMP diet from weaning through 20–22 wk of age. A: cells were gated on CD8 and plots are representative of mice fed either the casein or GMP diets. Histogram shows means ± SE percentage of CD8+ CD44hiCD62Llo cells, n = 8–18. B: percentage of CD8+ cells producing IFN-γ detected by intracellular cytokine staining.
