Fig. 7.

DNA methylation regulation. A: 16 genes were differentially methylated following AZT in WT and MCAT paired studies. Among these genes were PKCα. B: gene expression of PKCα from paired microarray analysis. Microarray expression for each biological replicate is normalized to vehicle-treated WT expression. *P < 0.05 compared with WT-Veh, #significant interaction (P < 0.05 by 2-way ANOVA) between MCAT and AZT. C: DNA methylation peak scores of PKCα with respect to genomic location for all 4 treatment groups. Each peak score represents the average of 4 arrays for each treatment group. Regions A, B, and C denote the 3 differentially methylated regions in the gene promoter. Note that PKCα lies on the negative strand, so the gene proceeds from the transcriptional start site to the left.