Table 5.
Genes differentially expressed (day −9 − day −24) in the mammary gland during the dry period enrich biofunctions related to milk production
| IPA Biofunctions |
||||||||
|---|---|---|---|---|---|---|---|---|
| Fold-change | P Value | LM | PS | VM | CM | RS | ||
| ACSM1* | acyl-CoA synthetase medium-chain 1 | 2.0 | 0.001 | X | ||||
| ALOX15 | arachidonate 15-lipoxygenase | 2.4 | < 0.001 | X | ||||
| ANGPTL4* | angiopoietin-like 4 | 2.1 | 0.019 | X | X | |||
| AQP1* | aquaporin 1 (Colton blood group) | 1.6 | 0.003 | X | ||||
| C3* | complement component 3 | 1.8 | 0.038 | X | X | X | X | |
| CAMP* | cathelicidin antimicrobial peptide | −2.8 | 0.003 | X | X | X | ||
| CD14* | CD14 molecule | 1.6 | 0.012 | X | X | X | ||
| CEBPD* | CCAAT/enhancer binding protein (C/EBP), delta | 1.7 | 0.036 | X | X | |||
| CFB | complement factor B | 2.4 | 0.025 | X | ||||
| CHI3L1* | chitinase 3-like 1 (cartilage glycoprotein-39) | 2.5 | 0.040 | X | ||||
| CIDEA* | cell death-inducing DFFA-like effector a | 2.3 | 0.004 | X | X | |||
| CP | ceruloplasmin (ferroxidase) | 2.7 | 0.002 | X | ||||
| CXCL10 | chemokine (C-X-C motif) ligand 10 | −1.7 | 0.008 | X | X | X | X | |
| CXCL13 | chemokine (C-X-C motif) ligand 13 | −2.1 | 0.033 | X | ||||
| CXCL2* | chemokine (C-X-C motif) ligand 2 | 3.0 | 0.018 | X | ||||
| CYP11A1 | cytochrome P450, family 11, subfamily A1 | 3.2 | 0.016 | X | X | X | ||
| DARC | Duffy blood group, chemokine receptor | 1.7 | 0.008 | X | ||||
| DUSP1* | dual specificity phosphatase 1 | 1.6 | 0.002 | X | X | X | ||
| FABP3* | fatty acid binding protein 3 | 3.0 | 0.001 | X | X | |||
| GK* | glycerol kinase | 1.6 | < 0.001 | X | X | |||
| HP | haptoglobin | 4.5 | 0.025 | X | ||||
| IGFBP5 | insulin-like growth factor binding protein 5 | 1.6 | 0.029 | X | X | X | ||
| IL13RA1 | interleukin 13 receptor, alpha 1 | 1.5 | 0.007 | X | ||||
| IL17RB* | interleukin 17 receptor B | −1.5 | 0.022 | X | ||||
| IL33 | interleukin 33 | 2.1 | 0.001 | X | X | |||
| IL6 | interleukin 6 | 1.7 | 0.002 | X | X | X | X | X |
| KLF6* | Kruppel-like factor 6 | 1.5 | 0.011 | X | ||||
| LALBA* | α-lactalbumin | 1.5 | 0.005 | X | X | |||
| LBP* | lipopolysaccharide binding protein | 3.4 | 0.005 | X | X | |||
| LPIN1 | lipin 1 | 1.7 | 0.023 | X | ||||
| LPL* | lipoprotein lipase | 1.9 | < 0.001 | X | X | X | ||
| MSTN | myostatin | 1.6 | < 0.001 | X | X | |||
| MUC1* | mucin 1, cell surface associated | 2.0 | 0.003 | X | ||||
| NT5E* | 5′-nucleotidase, ecto (CD73) | 1.6 | 0.031 | X | ||||
| NTS | neurotensin | 1.7 | 0.002 | X | X | |||
| PDK4* | pyruvate dehydrogenase kinase, isozyme 4 | 1.9 | 0.014 | X | X | X | ||
| PLVAP | plasmalemma vesicle | 1.6 | < 0.001 | X | X | |||
| PTHLH | parathyroid hormone-like hormone | 2.5 | 0.002 | X | X | X | ||
| RAB35* | Rab 35, member ras | 1.5 | 0.005 | X | X | |||
| RBP1 | retinol binding protein 1, cellular | 1.8 | 0.042 | X | X | |||
| S100A9* | S100 calcium binding protein A9 | 2.4 | 0.013 | X | ||||
| SDC2 | syndecan 2 | 1.5 | 0.005 | X | X | |||
| SFN | stratifin | 1.9 | 0.021 | X | X | |||
| SULF2 | sulfatase 2 | 1.6 | 0.027 | X | ||||
| THBD | thrombomodulin | 1.9 | 0.005 | X | X | |||
Biopsies were obtained from cows during the dry period on day −24 and −9 relative to parturition. Microarray analysis was conducted on purified RNA using Affymetrix GeneChip Bovine Genome Arrays. Genes were identified in the effect of time relative to parturition (day −9 − day −24). Differential expression was attributed to genes meeting the criteria of P ≤ 0.05 and fold-change ≥ |1.5|. IPA biofunctions - LM, lipid metabolism; PS, protein synthesis; VM, vitamin and mineral metabolism; CM, carbohydrate metabolism; RS, reproductive system development and function.
Genes in common with the bovine lactation genome (33).