Fig. 2.

Cytoplasmic VX-770 weakly potentiated hCFTR. Representative macropatch currents of hCFTR recorded in inside-out mode with symmetrical 150 mM Cl⁻ solution under the following experimental conditions with or without 10 μM VX-770: 1 mM MgATP + 127.6 U/ml PKA (A), 1 mM MgATP + 25.5 U/ml PKA (B), and 50 μM MgATP + 127.6 U/ml PKA (C). A voltage-ramp protocol described in the materials and methods section was applied every 5 s. D: summary data for fractional increase of hCFTR current by VX-770 under each set of conditions; n = 4–7 each.