Effect of O3 on pulmonary expression of ROCK1, ROCK2, and RhoA. A: Western blot showing ROCK1, ROCK2, RhoA, and β-actin expression. Protein was extracted from lungs of wild-type mice exposed to air or O3 (2 ppm for 3 h). B–D: densitometry for ROCK1 (B), ROCK2 (C), and RhoA (D) normalized for β-actin expression. Results are mean ± SE for 7 mice/group. Statistics were performed on log-transformed data to conform to a normal distribution.