Effect of ROCK inhibition in human airway smooth muscle (HASM) cells. Traction forces normalized to baseline values were assessed in HASM cells treated with various doses of the ROCK2 inhibitor SR3677 (A, C, and E), the pan-ROCK inhibitor Y27632 (B, D, and F), or an equal volume of water (the vehicle used to dissolve SR3677 and Y27632) and challenged with endothelin-1 (ET-1; A and B), histamine (C and D), or bradykinin (E and F) or vehicle. Data are from 2 HASM cell donors and are expressed as means ± SE of n = 9–21 wells/group for ET-1 and histamine, and n = 6–21 wells/group for bradykinin. *P < 0.05 vs. vehicle; #P < 0.05 vs. water control.