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. 2015 Oct 6;9:389. doi: 10.3389/fncel.2015.00389

Figure 2.

Figure 2

VNUT-immunoreactive cells co-localize with tyrosine-hydroxylase positive dopaminergic amacrine/interplexiform cells. Vertical cryostat sections of (A) adult mouse and (C) rat retinae expressed VNUT-IR in an amacrine/interplexiform cell (IPC) type in the INL. Pre-incubation of the VNUT antibody with excess peptide antigen resulted in no immunoreactivity, confirming the specificity of the antibody in the (B) mouse and (D) rat retinas. (E–J) VNUT immunoreactivity (green) was exclusively expressed in tyrosine hydroxylase-labeled dopaminergic amacrine/IPC cells and processes (red) as shown in a vertical section (E–G) and flatmounted mouse retina (H–J). Arrows (A, E–G) indicate interplexiform processes co-labeled with VNUT and TH extending toward the OPL. Arrowheads (A) indicate processes extending towards the IPL. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars = 20 μm.