FIG. 5.

Feeding status affects the periprandial expression of GOAT and preproghrelin mRNAs in the brain and gut of zebrafish. Pre- and postprandial expression of GOAT and preproghrelin mRNAs in the brain (A, B) and gut (C, D) of zebrafish. The expression of GOAT and preproghrelin mRNA was normalized to eef2a1, and represented relative to the expression in the −3 h group. Asterisks represent significant differences between groups at the same time point (p<0.05, n=6 zebrafish). Same letter (a) indicates no differences between time points tested, especially the preprandial sampling points, while changes observed at various times are indicated by different letters (b, c).