FIG. 7.

Ghrelin and GOAT precursor proteins are expressed in zebrafish gut and are affected by nutrient status. Fifty micrograms of total protein/well was loaded from the whole tissue lysates. Representative immunoblot shows bands representing preproghrelin corresponding to 16 kDa (A), GOAT corresponding to 50 kDa (B), and vinculin corresponding to 125 kDa. Average relative protein expression levels±SEM from four separate zebrafish gut per treatment group. Asterisk (*) indicates significant difference (p<0.05; n=4 zebrafish).