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. 2015 Oct 1;12(5):327–338. doi: 10.1089/zeb.2014.1062

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 8. — Effects of intraperitoneal injection of ghrelin on GOAT and preproghrelin mRNA expression in the brain and gut before and after regular feeding time. Intraperitoneal injection of ghrelin decreased expression of GOAT mRNA in zebrafish brain before and after regular feeding time (A) and zebrafish gut before regular feeding time (C). Intraperitoneal injection of ghrelin decreased expression of preproghrelin mRNA in zebrafish brain after regular feeding time (B). No changes were seen in zebrafish gut either before or after regular feeding time (D). GOAT and preproghrelin mRNA expression was normalized to eef2a1 and presented relative to the saline-injected group. Asterisks (*) represent significant differences between groups at the same time point (p<0.05, n=7 zebrafish).